5-TCO-PEG4-dUTP, 5-trans-Cyclooctene-PEG4-dUTP

Jena Bioscience

5-TCO-PEG4-dUTP, 5-trans-Cyclooctene-PEG4-dUTP

5

Research Use Only

1.0-1.1 mM

≥95%

C32H53N4O21P3 (free acid)

922.70 g/mol (free acid)

5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasksto introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins

C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCOCCOCCOCCOCCNC(=O)OC1CCCC=CCC1

-20°C

12352200