5-TCO-PEG4-dUTP

Biorbyt

5-TCO-PEG4-dUTP

16

Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: lambdamax 240 nm, epsilon 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

1.0-1.1 mM

Liquid

5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice: to introduce a Biotin group (via Tetrazines of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins.

-20°C

12352200