Bio-Connect

5-TCO-PEG4-dUTP

Research Use Only
ORB532289
Biorbyt
Product group Molecular Biology
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Overview

  • Supplier
    Biorbyt
  • Product Name
    5-TCO-PEG4-dUTP
  • Delivery Days Customer
    16
  • Application Supplier Note
    Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: lambdamax 240 nm, epsilon 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
  • Certification
    Research Use Only
  • Concentration
    1.0-1.1 mM
  • Formulation
    Liquid
  • Scientific Description
    5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice: to introduce a Biotin group (via Tetrazines of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352200