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Chemical Structure
Chemical Structure
Chemical Structure

6-Amino-D-luciferin [161055-47-6]

Research Use Only
CDX-A0306
Chemodex
CAS Number161055-47-6
Product group Chemicals
Estimated Purity>99%
Molecular Weight279.34
Price on request
Packing Size
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Overview

  • Supplier
    Chemodex
  • Product Name
    6-Amino-D-luciferin [161055-47-6]
  • Delivery Days Customer
    10
  • CAS Number
    161055-47-6
  • Certification
    Research Use Only
  • Estimated Purity
    >99%
  • Molecular Formula
    C11H9N3O2S2
  • Molecular Weight
    279.34
  • Scientific Description
    Amino analog of the common D-luciferin substrate for use in measuring firefly luciferase activity. This cell permeable reagent can be used when considering design of proluminescent bioconjugates (peptidase substrates) of luciferin for ultrasensitive luminescent assays. In bioluminescent protease assays peptides or amino acids are conjugated to the luciferin analog 6-amino-D-luciferin. These substrates are useful for ultrasensitive analysis of a variety of protease enzymes including various caspases and cathepsins, chymotrypsin, trypsin, elastase, kallikrein, thrombin, ficin, bromoalain, plasmin, papain, ficin and many others or even for use in vivo tracing of luciferase transfected cells in living tissues. The resulting bioluminescence spectrum of reaction of 6-Amino-D-luciferin is very similar to that observed for the natural substrate D-luciferin. Peptidase assays using a bioluminogenic substrate can be easily adapted to 96-well formats for HTS-based assays. Using this principle, it is possible to design an assay to detect almost any peptidase or protease activity via bioluminescence by using a substrate with a suitable peptide sequence. Applications in pathogen detection, discovery of protease inhibitors, probing cell physiology and assessing protease activity in oncogenesis are possible at extraordinary sensitivity. - Chemical. CAS: 161055-47-6. Formula: C11H9N3O2S2. MW: 279.34. Synthetic. Amino analog of the common D-luciferin substrate for use in measuring firefly luciferase activity. This cell permeable reagent can be used when considering design of proluminescent bioconjugates (peptidase substrates) of luciferin for ultrasensitive luminescent assays. In bioluminescent protease assays peptides or amino acids are conjugated to the luciferin analog 6-amino-D-luciferin. These substrates are useful for ultrasensitive analysis of a variety of protease enzymes including various caspases and cathepsins, chymotrypsin, trypsin, elastase, kallikrein, thrombin, ficin, bromoalain, plasmin, papain, ficin and many others or even for use in vivo tracing of luciferase transfected cells in living tissues. The resulting bioluminescence spectrum of reaction of 6-Amino-D-luciferin is very similar to that observed for the natural substrate D-luciferin. Peptidase assays using a bioluminogenic substrate can be easily adapted to 96-well formats for HTS-based assays. Using this principle, it is possible to design an assay to detect almost any peptidase or protease activity via bioluminescence by using a substrate with a suitable peptide sequence. Applications in pathogen detection, discovery of protease inhibitors, probing cell physiology and assessing protease activity in oncogenesis are possible at extraordinary sensitivity.
  • SMILES
    NC1=CC2=C(C=C1)N=C(S2)C1=N[C@H](CS1)C(O)=O
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352200