6-Azido-Trehalose, 6-TreAz

Jena Bioscience

6-Azido-Trehalose, 6-TreAz

5

Research Use Only

≥90%

C12H21N3O10

367.31 g/mol

Myobacteria are characterized by a trehalose-lipopolysaccharide-containing outer cell membrane. The Azide-functionalized 6-Azido-Trehalose provides an attractive approach for the detection of Myobacteria in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart. The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Myobacteria strains tested[1]: Mycobacterium smegmatis mc2155, Mycobacterium tuberculosis H37Rv,Mycobacterium bovis BCG

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-20°C

12352200