Western Blot Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng All lanes: HIS antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng All lanes: HIS antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
6His Monoclonal Antibody
CSB-MA000011M0M
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityAll Species
Overview
- SupplierCusabio
- Product Name6His Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3G5H8
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionA His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
- ReactivityAll Species
- Storage Instruction-20°C or -80°C
- UNSPSC12352203