9-Amino-6-chloro-2-methoxyacridine [3548-09-2] [3548-09-2]

Chemodex

9-Amino-6-chloro-2-methoxyacridine [3548-09-2] [3548-09-2]

10

3548-09-2

Research Use Only

>95%

Warning

C14H11ClN2O

258.7

9-Amino-6-chloro-2-methoxyacridine (ACMA) is a cell-permeable ph-sensitive fluorescent probe that intercalates into DNA. It selectively binds to poly(dA-dT) sequences with the fluorescence lifetime decreasing with incorporation of guanosine. It is used for nucleic acid staining/labeling. ACMA fluorescence is pH-dependent and is quenched when a pH gradient is established. Spectral Data: lambdaex 411nm; lambdaem 475nm (in methanol). Excitation of the ACMA-DNA complex (excitation/emission maxima lambda419/483 nm) is possible with most UV-light sources, making it compatible for use with both shorter- and longer-wavelength dyes. ACMA also apparently binds to membranes in the energized state and becomes quenched if a pH gradient forms. It has been extensively employed to follow cation and anion movement across membranes and to study the proton-pumping activity of various membrane-bound ATPases. ACMA also inhibits acetylcholinesterase. - Chemical. CAS: 02.09.3548. Formula: C14H11ClN2O. MW: 258.7. 9-Amino-6-chloro-2-methoxyacridine (ACMA) is a cell-permeable ph-sensitive fluorescent probe that intercalates into DNA. It selectively binds to poly(dA-dT) sequences with the fluorescence lifetime decreasing with incorporation of guanosine. It is used for nucleic acid staining/labeling. ACMA fluorescence is pH-dependent and is quenched when a pH gradient is established. Spectral Data: lambdaex 411nm; lambdaem 475nm (in methanol). Excitation of the ACMA-DNA complex (excitation/emission maxima lambda419/483 nm) is possible with most UV-light sources, making it compatible for use with both shorter- and longer-wavelength dyes. ACMA also apparently binds to membranes in the energized state and becomes quenched if a pH gradient forms. It has been extensively employed to follow cation and anion movement across membranes and to study the proton-pumping activity of various membrane-bound ATPases. ACMA also inhibits acetylcholinesterase.

ClC1=CC=C(C(N)=C(C=C(OC)C=C2)C2=N3)C3=C1

-20°C,2°C to 8°C

12162000