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ProductsBack/A4 / PLP2 Antibody

A4 / PLP2 Antibody

LS-C663496
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPLP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    A4 / PLP2 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (5 - 20 µg/ml), IP, WB (0.2 - 2 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5355
  • Target name
    PLP2
  • Target description
    proteolipid protein 2
  • Target synonyms
    A4, A4LSB, proteolipid protein 2, A4 differentiation-dependent protein, differentiation-dependent protein A4, intestinal membrane A4 protein, proteolipid protein 2 (colonic epithelium-enriched)
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLP2 using anti-PLP2 antibody (A06255-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLP2 antigen affinity purified polyclonal antibody (Catalog # A06255-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLP2 at approximately 17 kDa. The expected band size for PLP2 is at 17 kDa.
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