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Western Blot analysis of COLO205 cells using ABCB10 Polyclonal Antibody
Western Blot analysis of COLO205 cells using ABCB10 Polyclonal Antibody
Western Blot analysis of COLO205 cells using ABCB10 Polyclonal Antibody

ABCB10 Antibody

CSB-PA000782
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABCB10
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCB10 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23456
  • Target name
    ABCB10
  • Target description
    ATP binding cassette subfamily B member 10
  • Target synonyms
    EST20237, M-ABC2, MTABC2, ATP-binding cassette sub-family B member 10, mitochondrial, ABC transporter 10 protein, ABC-me protein, ABC-mitochondrial erythroid protein, ATP-binding cassette transporter 10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, mitochondrial ATP-binding cassette 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NRK6
  • Protein Name
    ATP-binding cassette sub-family B member 10, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ABCB10 using anti-ABCB10 antibody (PB9971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: COLO320 whole cell lysates, Lane 3: 22RV1 whole cell lysates, Lane 4: PANC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB10 antigen affinity purified polyclonal antibody (Catalog # PB9971) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB10 at approximately 79 kDa, 65 kDa. The expected band size for ABCB10 is at 79 kDa.
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