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Western Blot analysis of 3T3 cells using MRP2 Polyclonal Antibody
Western Blot analysis of 3T3 cells using MRP2 Polyclonal Antibody
Western Blot analysis of 3T3 cells using MRP2 Polyclonal Antibody

ABCC2 Antibody

CSB-PA007024
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetABCC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1244
  • Target name
    ABCC2
  • Target description
    ATP binding cassette subfamily C member 2
  • Target synonyms
    ABC30; ATP-binding cassette sub-family C member 2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2; canalicular multidrug resistance protein; canalicular multispecific organic anion transporter 1; CMOAT; cMRP; DJS; MRP2; multidrug resistance-associated protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92887
  • Protein Name
    Canalicular multispecific organic anion transporter 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP2/ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP2/ABCC2 at approximately 170-250 kDa. The expected band size for MRP2/ABCC2 is at 174 kDa.
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