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Western blot All lanes: ABCE1 antibody at 4ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: k562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: ABCE1 antibody at 4ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: k562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: ABCE1 antibody at 4ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: k562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa

ABCE1 Antibody

CSB-PA001076ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABCE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6059
  • Target name
    ABCE1
  • Target description
    ATP binding cassette subfamily E member 1
  • Target synonyms
    ABC38, OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein, ATP-binding cassette, sub-family E (OABP), member 1, RNase L inhibitor 1, huHP68, ribonuclease 4 inhibitor, ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61221
  • Protein Name
    ATP-binding cassette sub-family E member 1
  • Scientific Description
    Antagonizes the binding of 2-5A (5-phosphorylated 2,5-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of ABCE1 using anti-ABCE1 antibody (A04173). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse 4T1 whole cell lysates, Laen 8: mouse NIH/3T3 whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (Catalog # A04173) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.
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