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WB analysis of various samples using GTX12303 ABI2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : human Placenta tissue lysate Lane 3 : MCF-7 cell lysate Lane 4 : HeLa cell lysate Lane 5 : Jurkat cell lysate
WB analysis of various samples using GTX12303 ABI2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : human Placenta tissue lysate Lane 3 : MCF-7 cell lysate Lane 4 : HeLa cell lysate Lane 5 : Jurkat cell lysate
WB analysis of various samples using GTX12303 ABI2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : human Placenta tissue lysate Lane 3 : MCF-7 cell lysate Lane 4 : HeLa cell lysate Lane 5 : Jurkat cell lysate

ABI2 antibody

GTX12303
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetABI2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ABI2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID10152
  • Target name
    ABI2
  • Target description
    abl interactor 2
  • Target synonyms
    ABI-2, ABI2B, AIP-1, AIP1, AblBP3, SSH3BP2, argBP1, argBPIA, argBPIB, abl interactor 2, abelson interactor 2, abl binding protein 3, abl-interacting protein 1 (SH3-containing protein), abl-interactor protein 2b, arg protein tyrosine kinase-binding protein, arg-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYB9
  • Protein Name
    Abl interactor 2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABI2 using anti-ABI2 antibody (A04302). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI2 antigen affinity purified polyclonal antibody (Catalog # A04302) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABI2 at approximately 56-70 kDa. The expected band size for ABI2 is at 56 kDa.
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