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ABI2 Antibody (YA2840)

HY-P83095
MedChem Express
TargetABI2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ABI2 Antibody (YA2840)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10152
  • Target name
    ABI2
  • Target description
    abl interactor 2
  • Target synonyms
    abelson interactor 2; ABI-2; ABI2B; abl binding protein 3; abl interactor 2; AblBP3; abl-interacting protein 1 (SH3-containing protein); abl-interactor protein 2b; AIP1; AIP-1; arg protein tyrosine kinase-binding protein; arg-binding protein 1; argBP1; argBPIA; argBPIB; SSH3BP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ABI2 Antibody (YA2840) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2840), targeting ABI2, with a predicted molecular weight of 56 kDa (observed band size: 75 kDa). ABI2 Antibody (YA2840) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABI2 using anti-ABI2 antibody (A04302). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI2 antigen affinity purified polyclonal antibody (Catalog # A04302) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABI2 at approximately 56-70 kDa. The expected band size for ABI2 is at 56 kDa.
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