Ac4GlcNAz

Biorbyt

Ac4GlcNAz [98924-81-3]

10

Glycoconjugate synthesis monitoring by metabolic labeling.

98924-81-3

Research Use Only

Theoretical MW: 430.37 g/mol; Detected MW: 430.13 g/mol

The tetraacetylated N-Azidoacetyl-glucosamine (Ac 4GlcNAz) provides a non-radioactive alternative for glycoconjugate vi-sualization. It is cell-permeable, intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylglucosamine(GlcNAc). The resulting Azide-functionalized glycoconjugates can subse-quently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Recommended concentration for metabolic labeling: 25-75 microM. This concentration range may serve as a starting point for an individual assay set-up.

-20°C

12352200