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Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue All lanes: ACAA2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue All lanes: ACAA2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue All lanes: ACAA2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa

ACAA2 Antibody

CSB-PA001118LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACAA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACAA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10449
  • Target name
    ACAA2
  • Target description
    acetyl-CoA acyltransferase 2
  • Target synonyms
    3-ketoacyl-CoA thiolase, mitochondrial; acetyl-CoA acetyltransferase; acetyl-Coenzyme A acyltransferase 2; acyl-CoA hydrolase, mitochondrial; beta ketothiolase; DSAEC; mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; T1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42765
  • Protein Name
    3-ketoacyl-CoA thiolase, mitochondrial
  • Scientific Description
    Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACAA2 using anti-ACAA2 antibody (PB10022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: human Hela whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates, Lane 9: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAA2 antigen affinity purified polyclonal antibody (Catalog # PB10022) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAA2 at approximately 42 kDa. The expected band size for ACAA2 is at 42 kDa.
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