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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001126ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001126ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001126ESR2HU at dilution of 1:100

ACADM Antibody

CSB-PA001126ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACADM
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACADM Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID34
  • Target name
    ACADM
  • Target description
    acyl-CoA dehydrogenase medium chain
  • Target synonyms
    ACAD1, MCAD, MCADH, medium-chain specific acyl-CoA dehydrogenase, mitochondrial, acyl-CoA dehydrogenase, C-4 to C-12 straight chain, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, medium-chain acyl-CoA dehydrogenase, testicular tissue protein Li 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11310
  • Protein Name
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Scientific Description
    This enzyme is specific for acyl chain lengths of 4 to 16.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM/MCAD antigen affinity purified polyclonal antibody (Catalog # A02383-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADM/MCAD at approximately 47 kDa. The expected band size for ACADM/MCAD is at 47 kDa.
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