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WB analysis of various sample lysates using GTX32421 ACADM antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32421 ACADM antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32421 ACADM antibody. Dilution : 1:1000 Loading : 25microg per lane

ACADM antibody

GTX32421
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetACADM
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACADM antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID34
  • Target name
    ACADM
  • Target description
    acyl-CoA dehydrogenase medium chain
  • Target synonyms
    ACAD1, MCAD, MCADH, medium-chain specific acyl-CoA dehydrogenase, mitochondrial, acyl-CoA dehydrogenase, C-4 to C-12 straight chain, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, medium-chain acyl-CoA dehydrogenase, testicular tissue protein Li 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11310
  • Protein Name
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Scientific Description
    This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Gonzalez-Hurtado E, Lee J, Choi J, et al. Fatty acid oxidation is required for active and quiescent brown adipose tissue maintenance and thermogenic programing. Mol Metab. 2018,7:45-56. doi: 10.1016/j.molmet.2017.11.004
    Read this paper
  • Gonzalez-Hurtado E, Lee J, Choi J, et al. Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction. Am J Physiol Endocrinol Metab. 2017,312(5):E381-E393. doi: 10.1152/ajpendo.00408.2016
    Read this paper

Datasheet

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Figure 1. Western blot analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM/MCAD antigen affinity purified polyclonal antibody (Catalog # A02383-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADM/MCAD at approximately 47 kDa. The expected band size for ACADM/MCAD is at 47 kDa.
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