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ACADM Antibody (YA1967)

HY-P82222
MedChem Express
TargetACADM
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ACADM Antibody (YA1967)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID34
  • Target name
    ACADM
  • Target description
    acyl-CoA dehydrogenase medium chain
  • Target synonyms
    ACAD1; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; MCAD; MCADH; medium-chain acyl-CoA dehydrogenase; medium-chain specific acyl-CoA dehydrogenase, mitochondrial; testicular tissue protein Li 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ACADM Antibody (YA1967) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1967), targeting ACADM, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). ACADM Antibody (YA1967) can be used for WB, ICC/IF experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM/MCAD antigen affinity purified polyclonal antibody (Catalog # A02383-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADM/MCAD at approximately 47 kDa. The expected band size for ACADM/MCAD is at 47 kDa.
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