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Western blot All lanes: Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody at 7ug/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
Western blot All lanes: Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody at 7ug/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
Western blot All lanes: Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody at 7ug/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa

ACADS Antibody

CSB-PA001127ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACADS
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACADS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID35
  • Target name
    ACADS
  • Target description
    acyl-CoA dehydrogenase short chain
  • Target synonyms
    ACAD3; acyl-CoA dehydrogenase, C-2 to C-3 short chain; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; butyryl-CoA dehydrogenase; epididymis secretory sperm binding protein; mitochondrial short-chain specific acyl-CoA dehydrogenase; SCAD; short-chain specific acyl-CoA dehydrogenase, mitochondrial; unsaturated acyl-CoA reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16219
  • Protein Name
    Short-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACADS/SCAD using anti-ACADS/SCAD antibody (A05028-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat NRK whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1/6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADS/SCAD antigen affinity purified polyclonal antibody (Catalog # A05028-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADS/SCAD at approximately 44 kDa. The expected band size for ACADS/SCAD is at 44 kDa.
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