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Western Blot Positive WB detected in: HEK293 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: ACADSB antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: ACADSB antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: ACADSB antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa

ACADSB Antibody

CSB-PA001128LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetACADSB
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACADSB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID36
  • Target name
    ACADSB
  • Target description
    acyl-CoA dehydrogenase short/branched chain
  • Target synonyms
    2-MEBCAD, ACAD7, SBCAD, short/branched chain specific acyl-CoA dehydrogenase, mitochondrial, 2-methyl branched chain acyl-CoA dehydrogenase, 2-methylbutyryl-coenzyme A dehydrogenase, acyl-Coenzyme A dehydrogenase, short/branched chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45954
  • Protein Name
    Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
  • Scientific Description
    Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SBCAD/ACADSB using anti-SBCAD/ACADSB antibody (A08652-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human T47D whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SBCAD/ACADSB antigen affinity purified polyclonal antibody (Catalog # A08652-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SBCAD/ACADSB at approximately 40-44KD. The expected band size for SBCAD/ACADSB is at 47KD.
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