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ACAT2 Antibody

LS-C831868
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetACAT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACAT2 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID39
  • Target name
    ACAT2
  • Target description
    acetyl-CoA acetyltransferase 2
  • Target synonyms
    acetoacetyl Coenzyme A thiolase; acetyl-CoA acetyltransferase, cytosolic; acetyl-CoA transferase-like protein; cytosolic acetoacetyl-CoA thiolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ACAT2 antibody (A03245-1). Overlay histogram showing HepG2 cells stained with A03245-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAT2 Antibody (A03245-1, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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