accura-expRACE KIT

Cosmo Bio USA

accura-expRACE KIT

16

Research Use Only

Next-generation RACE In RACE (Rapid Amplification of cDNA Ends) by the single-primer method* of this kit, the targeted cDNA is amplified by PCR with only a gene-specific primer using ds cDNA as a template. The mechanism is based on that the terminal region of the ds cDNA is partially denatured at 68 C for the extension reaction and that the linear DNA molecule tends to circularize. Upon reaching the 5 end of the template DNA, thermostable DNA polymerase switches templates to the 5 terminal region of the newly synthesized daughter strand at a certain probability and synthesizes DNA sequences complementary to the gene-specific primer. Using this daughter strand as a template, the targeted cDNA is amplified with only a gene- specific primer. Feature and Advantages: By using the accura-expRACE KIT, both 5 and 3 RACE can easily and efficiently be performed under simple conditions, such as RT-PCR. Long cDNA and rare cDNA unidentified previously can be isolated. The synthesized ds cDNA can be used as a cDNA library. You can perform ~400 screenings of a cDNA library by RACE. The cDNA synthesized by this kit contains a high proportion of full-length cDNA because an advanced type M-MLV Reverse Transcriptase is used.

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