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ProductsBack/ACE antibody [9B21]

ACE antibody [9B21]

GTX53158
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetAce
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACE antibody [9B21]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9B21
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID11421
  • Target name
    Ace
  • Target description
    angiotensin I converting enzyme
  • Target synonyms
    CD143, angiotensin-converting enzyme, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, dipeptidyl carboxypeptidase I, dipeptidyl peptidase, kininase II
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP09470
  • Protein Name
    Angiotensin-converting enzyme
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ace using anti-Ace antibody (A00251). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ace antigen affinity purified polyclonal antibody (Catalog # A00251) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ace at approximately 180KD. The expected band size for Ace is at 150KD.
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