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Mouse kidney Probed with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse kidney Probed with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse kidney Probed with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

ACACA Recombinant Antibody

BSM-52486R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACACB
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Overview

  • Supplier
    Bioss
  • Product Name
    ACACA Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID32
  • Target name
    ACACB
  • Target description
    acetyl-CoA carboxylase beta
  • Target synonyms
    ACACbeta, ACC-beta, ACC2, ACCB, ACCbeta, HACC275, acetyl-CoA carboxylase 2, acetyl-Coenzyme A carboxylase beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00763
  • Protein Name
    Acetyl-CoA carboxylase 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACACB using anti-ACACB antibody (A03668-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates Lane 2: mouse skeletal muscle tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACB antigen affinity purified polyclonal antibody (Catalog # A03668-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACB at approximately 277KD. The expected band size for ACACB is at 277KD.
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