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Acetyl Coenzyme A Carboxylase Antibody

HY-P80528
MedChem Express
TargetACACB
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Acetyl Coenzyme A Carboxylase Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID32
  • Target name
    ACACB
  • Target description
    acetyl-CoA carboxylase beta
  • Target synonyms
    ACC2; ACCB; ACC-beta; acetyl-CoA carboxylase 2; acetyl-Coenzyme A carboxylase beta; HACC275
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Acetyl Coenzyme A Carboxylase Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 277 kDa, targeting to Acetyl Coenzyme A Carboxylase. It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACACB using anti-ACACB antibody (A03668-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates Lane 2: mouse skeletal muscle tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACB antigen affinity purified polyclonal antibody (Catalog # A03668-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACB at approximately 277KD. The expected band size for ACACB is at 277KD.
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