ACHE Colorimetric Assay Kit

BPS Bioscience

ACHE Colorimetric Assay Kit

7

Research Use Only

AChE

The ACHE Colorimetric Assay Kit is designed to measure the activity of ACHE (acetylcholinesterase) for screening and profiling applications. The ACHE colorimetric reaction is based on a modified Ellmans method, using the alternative substrate acetylthiocholine iodide (ATCI) and 5,5-dithio-bis-2-nitrobenzoic acid (DTNB) to quantify amount of thiocholine produced from the hydrolysis of ATCI by ACHE. This assay kit comes in a convenient 96-well format, with purified ACHE enzyme (amino acids 32-614), ATCI, DTNB and assay buffer for 100 enzyme reactions. The absorption intensity of DTNB adduct (lambda=410 nm) is used to measure the amount of thiocholine product generated, which is proportional to ACHE activity.

See manual

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