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WB analysis of Jurkat cells using GTX47703 AChR alpha 5 antibody at 1.0microg/ml.
WB analysis of Jurkat cells using GTX47703 AChR alpha 5 antibody at 1.0microg/ml.
WB analysis of Jurkat cells using GTX47703 AChR alpha 5 antibody at 1.0microg/ml.

AChR alpha 5 antibody, Internal

GTX47703
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCHRNA5
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Overview

  • Supplier
    GeneTex
  • Product Name
    AChR alpha 5 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1138
  • Target name
    CHRNA5
  • Target description
    cholinergic receptor nicotinic alpha 5 subunit
  • Target synonyms
    acetylcholine receptor, nicotinic, alpha 5 (neuronal); Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5; cholinergic receptor, nicotinic alpha 5; cholinergic receptor, nicotinic, alpha 5 (neuronal); LNCR2; neuronal acetylcholine receptor subunit alpha-5; neuronal nicotinic acetylcholine receptor, alpha5 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30532
  • Protein Name
    Neuronal acetylcholine receptor subunit alpha-5
  • Scientific Description
    The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat skeletal muscle tissue lysates, lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA5 antigen affinity purified polyclonal antibody (Catalog # A02359-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRNA5 at approximately 53KD. The expected band size for CHRNA5 is at 53KD.
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