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ProductsBack/ACP5 / TRAP Antibody (clone ABT-TRAP)

ACP5 / TRAP Antibody (clone ABT-TRAP)

LS-C744576
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetACP5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACP5 / TRAP Antibody (clone ABT-TRAP)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Gaint cell tumor. Resulting localization is Cytoplasmic
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Gaint cell tumor. Resulting localization is Cytoplasmic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABT-TRAP
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54
  • Target name
    ACP5
  • Target description
    acid phosphatase 5, tartrate resistant
  • Target synonyms
    HPAP; human purple acid phosphatase; tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5a; tartrate-resistant acid phosphatase 5b; tartrate-resistant acid phosphatase type 5; TRACP5a; TRACP5b; TRAP; TrATPase
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (A03277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid Phosphatase/ACP5 antigen affinity purified polyclonal antibody (Catalog # A03277-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid Phosphatase/ACP5 at approximately 39 kDa. The expected band size for Acid Phosphatase/ACP5 is at 37 kDa.
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