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Immunofluorescent analysis of HepG2 cells using CSB-PA001191LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using CSB-PA001191LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using CSB-PA001191LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ACSL1 Antibody

CSB-PA001191LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetACSL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACSL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2180
  • Target name
    ACSL1
  • Target description
    acyl-CoA synthetase long chain family member 1
  • Target synonyms
    ACS1, FACL1, FACL2, LACS, LACS1, LACS2, long-chain-fatty-acid--CoA ligase 1, LACS 1, LACS 2, acyl-CoA synthetase 1, arachidonate--CoA ligase, fatty-acid-Coenzyme A ligase, long-chain 1, fatty-acid-Coenzyme A ligase, long-chain 2, lignoceroyl-CoA synthase, long-chain acyl-CoA synthetase 1, long-chain acyl-CoA synthetase 2, long-chain fatty acid-CoA ligase 2, long-chain fatty-acid-coenzyme A ligase 1, palmitoyl-CoA ligase 1, palmitoyl-CoA ligase 2, paltimoyl-CoA ligase 1, phytanate--CoA ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33121
  • Protein Name
    Long-chain-fatty-acid--CoA ligase 1
  • Scientific Description
    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACSL1 using anti-ACSL1 antibody (PB10025). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL1 antigen affinity purified polyclonal antibody (Catalog # PB10025) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSL1 at approximately 78 kDa. The expected band size for ACSL1 is at 78 kDa.
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