Activin A: Activin RIIB[Biotinylated] Inhibitor Screening Colorimetric Assay Kit

BPS Bioscience

Activin A: Activin RIIB[Biotinylated] Inhibitor Screening Colorimetric Assay Kit

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Research Use Only

Biotin

The Activin A: Activin RIIB [Biotinylated] Inhibitor Screening Colorimetric Assay Kit is a colorimetric-based assay designed to measure the binding between human Activin A and Activin RIIB (receptor IIB), also known as ACVR2B, for screening and profiling applications. This kit comes with enough purified Activin A (amino acids 311-426) and biotinylated Activin Receptor IIB (amino acids 19-137), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. Activin A: Activin RIIB[Biotinylated] Inhibitor Screening Colorimetric Assay Kit schematic. A 96-well plate is coated with Activin A protein. After blocking, biotinylated Activin RIIB (ACVR2B ) is added in an optimized assay buffer. Next, unbound biotinylated Activin RIIB is removed by washing, and the plate is incubated with streptavidin-HRP. After a final wash, colorimetric HRP substrate is added to produce color, which is proportional to the binding of ACVR2B with Activin A.

See manual

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