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ACTN3 Antibody

LS-C401167
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetACTN3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACTN3 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID89
  • Target name
    ACTN3
  • Target description
    actinin alpha 3
  • Target synonyms
    ACTN3D; alpha-actinin skeletal muscle; alpha-actinin-3; F-actin cross-linking protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunofluorescence staining of Hela cells with CSB-PA001243LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody (PB10026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: mouse skeletal muscle tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN3/Alpha Actinin 3 antigen affinity purified polyclonal antibody (Catalog # PB10026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN3/Alpha Actinin 3 at approximately 103 kDa. The expected band size for ACTN3/Alpha Actinin 3 is at 103 kDa.
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