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Western Blot analysis of K562 cells using Arp3 Polyclonal Antibody
Western Blot analysis of K562 cells using Arp3 Polyclonal Antibody
Western Blot analysis of K562 cells using Arp3 Polyclonal Antibody

ACTR3 Antibody

CSB-PA000932
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACTR3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACTR3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10096
  • Target name
    ACTR3
  • Target description
    actin related protein 3
  • Target synonyms
    actin-like protein 3; actin-related protein 3; ARP3; ARP3 actin related protein 3 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61158
  • Protein Name
    Actin-related protein 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Arp3/ACTR3 using anti-Arp3/ACTR3 antibody (A03423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole lysates, Lane 3: human U20S wholel cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mose spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arp3/ACTR3 antigen affinity purified polyclonal antibody (Catalog # A03423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arp3/ACTR3 at approximately 47KD. The expected band size for Arp3/ACTR3 is at 47KD.
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