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ProductsBack/ACVR2A Antibody - N-terminal region (OAAB16873)

ACVR2A Antibody - N-terminal region (OAAB16873)

OAAB16873
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACVR2A
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ACVR2A Antibody - N-terminal region (OAAB16873)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. PBS with 0.09% (W/V) sodium azide.
  • Gene ID92
  • Target name
    ACVR2A
  • Target description
    activin A receptor type 2A
  • Target synonyms
    activin A receptor, type IIA; activin receptor type-2A; ACTRII; ACVR2
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACVR2A using anti-ACVR2A antibody (A04770-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2A antigen affinity purified polyclonal antibody (Catalog # A04770-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR2A at approximately 58 kDa. The expected band size for ACVR2A is at 58 kDa.
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