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Immunohistochemical staining of human skin shows moderate to strong membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows moderate to strong membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows moderate to strong membranous positivity in squamous epithelial cells.

Anti-ACVR2A Antibody

HPA046997
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACVR2A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACVR2A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92
  • Target name
    ACVR2A
  • Target description
    activin A receptor type 2A
  • Target synonyms
    ACTRII, ACVR2, activin receptor type-2A, activin A receptor, type IIA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27037
  • Protein Name
    Activin receptor type-2A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ACVR2A using anti-ACVR2A antibody (A04770-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2A antigen affinity purified polyclonal antibody (Catalog # A04770-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR2A at approximately 58 kDa. The expected band size for ACVR2A is at 58 kDa.
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