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ADAM15 Antibody (clone 1B7G8, Preservative Free)

LS-C819576
LifeSpan BioSciences
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
TargetADAM15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ADAM15 Antibody (clone 1B7G8, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ELISA
  • Applications Supplier
    ELISA (1:1000 - 1:2000), Flo (1:25 - 1:100) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1B7G8
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8751
  • Target name
    ADAM15
  • Target description
    ADAM metallopeptidase domain 15
  • Target synonyms
    a disintegrin and metalloproteinase domain 15 (metargidin); disintegrin and metalloproteinase domain-containing protein 15; MDC15; MDC-15; metalloprotease RGD disintegrin protein; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM15 using anti-ADAM15 antibody (A02593-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM15 antigen affinity purified polyclonal antibody (Catalog # A02593-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM15 at approximately 100KD. The expected band size for ADAM15 is at 93KD.
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