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ADAM19 antibody

GTX11550
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetADAM19
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Overview

  • Supplier
    GeneTex
  • Product Name
    ADAM19 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8728
  • Target name
    ADAM19
  • Target description
    ADAM metallopeptidase domain 19
  • Target synonyms
    a disintegrin and metalloproteinase domain 19 (meltrin beta); disintegrin and metalloproteinase domain-containing protein 19; FKSG34; MADDAM; metalloprotease and disintegrin dendritic antigen marker; metalloprotease-disintegrin meltrin beta; MLTNB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H013
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 19
  • Scientific Description
    The ADAM19 gene encodes a disintegrin and metalloprotease (ADAM) domain 19, which is a member of the ADAM protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Alternative splicing generates 2 transcript variants for this gene.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM19 using anti-ADAM19 antibody (PA2070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM19 antigen affinity purified polyclonal antibody (Catalog # PA2070) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM19 at approximately 105 kDa. The expected band size for ADAM19 is at 105 kDa.
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