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ADAM28 Antibody

LS-C749829
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetADAM28
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ADAM28 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 61kDa/87kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 61kDa/87kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10863
  • Target name
    ADAM28
  • Target description
    ADAM metallopeptidase domain 28
  • Target synonyms
    ADAM 28, MDC-L, MDCL, eMDC II, eMDCII, disintegrin and metalloproteinase domain-containing protein 28, epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II, epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II, epididymis secretory sperm binding protein, metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM28 using anti-ADAM28 antibody (A06873-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysates,lane 2: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM28 antigen affinity purified polyclonal antibody (Catalog # A06873-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM28 at approximately 87/61KD. The expected band size for ADAM28 is at 87KD.
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