Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_T-45075_20230707_WB_Fraction_23071223_875.webp "Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_T-45075_20230811_WB_shRNA_watermark_23081619_744.webp "Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20230908_WB_D_23091319_905.webp "Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20230922_WB_Fraction_23100118_931.webp "SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20240223_WB_shRNA_competitor_watermark_24022619_670.webp "Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:2000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20240301_WB_Fraction_competitor_watermark_24030600_600.webp "Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:2000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20250117_IHC-P_M_25012200_362.webp "Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20250117_IHC-P_M_Array_25012200_365.webp "Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20250117_IHC-P_R_25012200_464.webp "Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Adenosine A1 Receptor antibody [HL2442]
GTX638758
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetADORA1
Overview
- SupplierGeneTex
- Product NameAdenosine A1 Receptor antibody [HL2442]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2442
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID134
- Target nameADORA1
- Target descriptionadenosine A1 receptor
- Target synonymsRDC7, adenosine receptor A1
- HostRabbit
- IsotypeIgG
- Protein IDP30542
- Protein NameAdenosine receptor A1
- Scientific DescriptionThe protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5 UTR have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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