
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Adenylate kinase 4(GTX107482) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Adenylate kinase 4 antibody [N1C3-2]
GTX107482
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAK4
Overview
- SupplierGeneTex
- Product NameAdenylate kinase 4 antibody [N1C3-2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID205
- Target nameAK4
- Target descriptionadenylate kinase 4
- Target synonymsAK 4, AK3, AK3L1, AK3L2, adenylate kinase 4, mitochondrial, ATP-AMP transphosphorylase, GTP:AMP phosphotransferase AK4, mitochondrial, adenylate kinase 3-like 1, adenylate kinase isoenzyme 4, mitochondrial, epididymis secretory sperm binding protein, mitochondrial adenylate kinase-3, nucleoside-triphosphate-adenylate kinase
- HostRabbit
- IsotypeIgG
- Protein IDP27144
- Protein NameAdenylate kinase 4, mitochondrial
- Scientific DescriptionThis gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Jan YH, Lai TC, Yang CJ, et al. A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma. Sci Rep. 2019,9(1):12329. doi: 10.1038/s41598-019-48243-9Read this paper


![Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Adenylate kinase 4 protein stained by Adenylate kinase 4 antibody [N1C3-2] (GTX107482) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm. Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Adenylate kinase 4 protein stained by Adenylate kinase 4 antibody [N1C3-2] (GTX107482) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107482/GTX107482_40660_IFA_w_23060120_384.webp)
![Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Adenylate kinase 4 antibody [N1C3-2] (GTX107482) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Adenylate kinase 4 antibody [N1C3-2] (GTX107482) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107482/GTX107482_40660_20150806_IHC-P_M_w_23060120_901.webp)





![Adenylate kinase 4 antibody [N1C3] detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Adenylate kinase 4 protein stained by Adenylate kinase 4 antibody [N1C3] (GTX104430) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104430/GTX104430_39953_IFA_w_23060120_811.webp)