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IHC-P analysis of human adenal carcinoma tissue section using GTX03304 ADFP antibody [DBM15.60].
IHC-P analysis of human adenal carcinoma tissue section using GTX03304 ADFP antibody [DBM15.60].
IHC-P analysis of human adenal carcinoma tissue section using GTX03304 ADFP antibody [DBM15.60].

ADFP antibody [DBM15.60]

GTX03304
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPLIN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ADFP antibody [DBM15.60]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DBM15.60
  • Conjugate
    Unconjugated
  • Gene ID123
  • Target name
    PLIN2
  • Target description
    perilipin 2
  • Target synonyms
    ADFP; adipophilin; adipose differentiation-related protein; ADRP; perilipin-2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ99541
  • Protein Name
    Perilipin-2
  • Scientific Description
    The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADFP using anti-ADFP antibody (PB10083). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADFP antigen affinity purified polyclonal antibody (Catalog # PB10083) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADFP at approximately 48 kDa. The expected band size for ADFP is at 48 kDa.
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