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Adiponectin antibody

10-7815
Biosynth
Product group Antibodies
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    Adiponectin antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Lane 1: Mouse Breast tissue lysates; Lane 2: Rat Breast tissue lysates probed with Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.
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Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001). Adiponectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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