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WB analysis of various sample lysates using GTX65581 ADRM1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65581 ADRM1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65581 ADRM1 antibody. Dilution : 1:1000 Loading : 25microg per lane

ADRM1 antibody

GTX65581
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetADRM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ADRM1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:4000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11047
  • Target name
    ADRM1
  • Target description
    ADRM1 26S proteasome ubiquitin receptor
  • Target synonyms
    110 kDa cell membrane glycoprotein; adhesion regulating molecule 1; ARM1; ARM-1; GP110; M(r) 110,000 surface antigen; proteasomal ubiquitin receptor ADRM1; proteasome regulatory particle non-ATPase 13; proteasome ubiquitin receptor; PSMD16; rpn13 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16186
  • Protein Name
    Proteasomal ubiquitin receptor ADRM1
  • Scientific Description
    This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADRM1/ARM-1 using anti-ADRM1/ARM-1 antibody (A04010-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRM1/ARM-1 antigen affinity purified polyclonal antibody (Catalog # A04010-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRM1/ARM-1 at approximately 42 kDa. The expected band size for ADRM1/ARM-1 is at 42 kDa.
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