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Western blot analysis of extracts of various cell lines using ADRM1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using ADRM1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using ADRM1 Polyclonal Antibody at dilution of 1:1000.

ADRM1 Polyclonal Antibody

E-AB-63791
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ADRM1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADRM1/ARM-1 using anti-ADRM1/ARM-1 antibody (A04010-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRM1/ARM-1 antigen affinity purified polyclonal antibody (Catalog # A04010-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRM1/ARM-1 at approximately 42 kDa. The expected band size for ADRM1/ARM-1 is at 42 kDa.
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