AEC

GTX30938
Product group Assays
Overview
- SupplierGeneTex
- Product NameAEC
- Delivery Days Customer9
- Application Supplier NoteTo 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. Add one drop of reagent C chromogen, mix well. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or countrys regulations. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/ deionized water. Add few drops of ready to use AEC reagent, incubate at RT or 37C for 5-10 minutes. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. Add counterstain compatible with AEC (we recommend hematoxylin counterstain for 30-60 seconds). Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
- ApplicationsImmunoHistoChemistry
- CertificationResearch Use Only
- FormulationLiquid
- Scientific DescriptionPeroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives bright red color and is very compatible with hematoxylin nuclear counterstain. This chromogen is soluble in alcohol and organic solvents therefore cannot be mounted with organic mounting medium. We recommend our aqueous mounting medium ImmunoHistoMount (IHM) for mounting of this chromogen.
- Storage Instruction2°C to 8°C
- UNSPSC41116133
References
- miR-155-deficient bone marrow promotes tumor metastasis. Yu F et al., 2013 Aug, Mol Cancer ResRead more