AF6-RBDGST (residues 1-141), GTP-H-Ras binding cytoplasmic protein, Afadin - Ras-binding domain, human, recombinant, E. coli

Jena Bioscience

AF6-RBDGST (residues 1-141), GTP-H-Ras binding cytoplasmic protein, Afadin - Ras-binding domain, human, recombinant, E. coli

5

Research Use Only

>90%

The amino terminus of Af6 was identified as the Rasbinding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.

-20°C

12352202