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The data was published in the journal Proteome Sci in 2013. PMID: 23802875
The data was published in the journal Proteome Sci in 2013. PMID: 23802875
The data was published in the journal Proteome Sci in 2013. PMID: 23802875

AFM antibody, Internal

GTX88333
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetAFM
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Overview

  • Supplier
    GeneTex
  • Product Name
    AFM antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.3-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID173
  • Target name
    AFM
  • Target description
    afamin
  • Target synonyms
    afamin; ALB2; ALBA; ALF; alpha-Alb; alpha-albumin
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP43652
  • Protein Name
    Afamin
  • Scientific Description
    This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms. Satoh K et al., 2013 Jun 27, Proteome Sci
    Read more

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Figure 1. Western blot analysis of AFM using anti-AFM antibody (A00275-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFM antigen affinity purified polyclonal antibody (Catalog # A00275-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFM at approximately 87KD. The expected band size for AFM is at 87KD.
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