Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
AFP (A2) Monoclonal Antibody
BSM-1621M
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetAFP
Overview
- SupplierBioss
- Product NameAFP (A2) Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:500-2000), ELISA(ELISA=1:5000-10000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDA2
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID174
- Target nameAFP
- Target descriptionalpha fetoprotein
- Target synonymsAFPD, FETA, HPAFP, alpha-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin
- HostMouse
- IsotypeIgG
- Protein IDP02771
- Protein NameAlpha-fetoprotein
- ReactivityHuman, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Liu X, Song X, Dong Z, et al. Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein. Biosens Bioelectron. 2017,91:431-435. doi: 10.1016/j.bios.2016.12.058Read this paper
- Zhang X, Tan X, Zhang B, et al. Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels. Anal Chem. 2016,88(13):6947-53. doi: 10.1021/acs.analchem.6b01821Read this paper
- Lin D, Mei C, Liu A, et al. Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition. Biosens Bioelectron. 2015,66:177-83. doi: 10.1016/j.bios.2014.10.074Read this paper
- Zhao C, Wu J, Ju H, et al. Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition. Anal Chim Acta. 2014,847:37-43. doi: 10.1016/j.aca.2014.07.035Read this paper
- Zong C, Wu J, Xu J, et al. Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging. Biosens Bioelectron. 2013,43:372-8. doi: 10.1016/j.bios.2012.12.051Read this paper
Datasheet
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