Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
AFP Monoclonal Antibody
BSM-1621M
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetAFP
Overview
- SupplierBioss
- Product NameAFP Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDA2
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID174
- Target nameAFP
- Target descriptionalpha fetoprotein
- Target synonymsAFPD, FETA, HPAFP, alpha-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin
- HostMouse
- IsotypeIgG
- Protein IDP02771
- Protein NameAlpha-fetoprotein
- ReactivityHuman, Rat
- Storage Instruction-20°C
- UNSPSC41116161
References
- Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein. Liu X et al., 2017 May 15, Biosens BioelectronRead this paper
- Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels. Zhang X et al., 2016 Jul 5, Anal ChemRead this paper
- Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition. Lin D et al., 2015 Apr 15, Biosens BioelectronRead this paper
- Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition. Zhao C et al., 2014 Oct 17, Anal Chim ActaRead this paper
- Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging. Zong C et al., 2013 May 15, Biosens BioelectronRead this paper
Datasheet
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