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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

ACAN Polyclonal Antibody

BS-11655R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACAN
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Overview

  • Supplier
    Bioss
  • Product Name
    ACAN Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID176
  • Target name
    ACAN
  • Target description
    aggrecan
  • Target synonyms
    AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, SSOAOD, aggrecan core protein, cartilage-specific proteoglycan core protein, chondroitin sulfate proteoglycan core protein 1, large aggregating proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16112
  • Protein Name
    Aggrecan core protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203