Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
ACAN Polyclonal Antibody
BS-11655R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACAN
Overview
- SupplierBioss
- Product NameACAN Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID176
- Target nameACAN
- Target descriptionaggrecan
- Target synonymsAGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, SSOAOD, aggrecan core protein, cartilage-specific proteoglycan core protein, chondroitin sulfate proteoglycan core protein 1, large aggregating proteoglycan
- HostRabbit
- IsotypeIgG
- Protein IDP16112
- Protein NameAggrecan core protein
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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