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ProductsBack/AGO4 / EIF2C4 Antibody (Preservative Free)

AGO4 / EIF2C4 Antibody (Preservative Free)

LS-C815219
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAGO4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AGO4 / EIF2C4 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID192670
  • Target name
    AGO4
  • Target description
    argonaute RISC component 4
  • Target synonyms
    argonaute 4, RISC catalytic component; argonaute RISC catalytic component 4; EIF2C4; eukaryotic translation initiation factor 2C, 4; protein argonaute-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Argonaute 4 using anti-Argonaute 4 antibody (PB9979). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Argonaute 4 antigen affinity purified polyclonal antibody (Catalog # PB9979) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Argonaute 4 at approximately 115 kDa. The expected band size for Argonaute 4 is at 97 kDa.
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