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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: AGPS antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: AGPS antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: AGPS antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa

AGPS Antibody

CSB-PA001457LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAGPS
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Overview

  • Supplier
    Cusabio
  • Product Name
    AGPS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8540
  • Target name
    AGPS
  • Target description
    alkylglycerone phosphate synthase
  • Target synonyms
    ADAP-S, ADAS, ADHAPS, ADPS, ALDHPSY, RCDP3, alkyldihydroxyacetonephosphate synthase, peroxisomal, aging-associated gene 5 protein, aging-associated protein 5, alkyl-DHAP synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00116
  • Protein Name
    Alkyldihydroxyacetonephosphate synthase, peroxisomal
  • Scientific Description
    Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AGPS using anti-AGPS antibody (A03481-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGPS antigen affinity purified polyclonal antibody (Catalog # A03481-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGPS at approximately 73 kDa. The expected band size for AGPS is at 73 kDa.
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