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ICC/IF analysis of HeLa cells using GTX22100 AHR antibody. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:100 Fixation : 10% formalin Permibilization : 0.5% Triton-X100
ICC/IF analysis of HeLa cells using GTX22100 AHR antibody. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:100 Fixation : 10% formalin Permibilization : 0.5% Triton-X100
ICC/IF analysis of HeLa cells using GTX22100 AHR antibody. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:100 Fixation : 10% formalin Permibilization : 0.5% Triton-X100

AHR antibody

GTX22100
GeneTex
ApplicationsGel Shift Assay, ImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAhr
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Overview

  • Supplier
    GeneTex
  • Product Name
    AHR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 5 - 20 microg/ml. IHC-P: 20 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Gel Shift Assay, ImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11622
  • Target name
    Ahr
  • Target description
    aryl-hydrocarbon receptor
  • Target synonyms
    A; Ah; ah receptor; Ahh; Ahre; aryl hydrocarbon receptor; bHLHe7; bHLHe76; dioxin receptor; In
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP30561
  • Protein Name
    Aryl hydrocarbon receptor
  • Scientific Description
    Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AHR using anti-AHR antibody (PB9419). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # PB9419) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 96 kDa. The expected band size for AHR is at 96 kDa.
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